In-Vitro Antibacterial Activity of Launaea procumbens Against Oral Pathogens Streptococcus mutans And Lactobacillus casei

Abstract

Background: The oral cavity is crucial for overall well-being but is susceptible to diseases due to bacteria forming dental plaque. Issues like cavities and gum diseases arise from these bacteria producing acids, weakening tooth enamel, and causing gum inflammation. Dentists use antibiotics to treat infections, but their excessive use leads to antibiotic resistance concerns. To combat this, dentistry is embracing herbal remedies with natural antimicrobial properties. By integrating these alternatives, dental practices can effectively manage oral infections while addressing global concerns about antibiotic resistance and promoting sustainable oral healthcare solutions.Top of Form

Objective: This research aims to assess the antibacterial properties of the traditionally used Medicinal Plant “Launaea procumbens” from Thar Desert, Sindh, against oral pathogens Streptococcus mutans and Lactobacillus casei. The study will compare their effectiveness with commonly used Cephalosporin, investigating potential solutions to bacterial resistance.

Methodology: To check the antibacterial activity the solvent extracts of Launaea procumbens (methanol, ethanol, and aqueous) were prepared and tested using the disk diffusion method. After incubation, the inhibition zones around the discs impregnated with plant extracts were measured and compared with the Cephalosporin. The experiment was repeated three times for each extract. The MIC assay was also conducted using serial dilutions and a growth indicator to determine the lowest concentration inhibiting bacterial growth visually.

Conclusion: The results indicated that the ethanolic and methanolic extracts exhibited significant inhibitory effects on both bacterial strains, highlighting their potential as effective inhibitors of these harmful bacteria